Human Semaphorin-5A (SEMA5A) ELISA Kit | KTE62333

Human Semaphorin-5A (SEMA5A) ELISA Kit | KTE62333 | Gentaur UK, US & Europe Distribution

Application: This Human Semaphorin-5A (SEMA5A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA5A in samples. An antibody specific for SEMA5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Semaphorin-5A microplate
• Human Semaphorin-5A standard
• Human Semaphorin-5A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Semaphorin-5A (SEMA5A) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA5A. No significant cross-reactivity or interference between Human SEMA5A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SEMA5A belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene. The deduced 1, 077-amino acid protein contains an N-terminal signal sequence, followed by a semaphorin domain, 7 thrombospondin type-1 repeats, a putative transmembrane domain, and a short cytoplasmic tail. It also has 13 conserved cysteines and a conserved N-glycosylation site. Northern blot analysis of whole mouse embryos detected 2 Semf transcripts at embryonic day 10, and their expression decreased toward birth.

Alternative Names: SEMA5A; FLJ12815; SEMAF; semF; sema domain; seven thrombospondin repeats (type 1 and type 1-like) ; transmembrane domain (TM) and short cytoplasmic domain; 5A; semaphorin 5A; semaphorin F

Search name: SEMA5A; FLJ12815; SEMAF; semF; sema domain; seven thrombospondin repeats (type 1 and type 1-like) ; transmembrane domain (TM) and short cytoplasmic domain; 5A; semaphorin 5A; semaphorin F

Tag: SEMA5A