Human Semenogelin-2 (SEMG2) ELISA Kit | AE20150HU

Human Semenogelin-2 (SEMG2) ELISA Kit | AE20150HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SEMG2

Alternative Name: SGII; Semenogelin 2

Application: ELISA

Range: 0.78-50 ng/mL

Sensitivity: 0.28 ng/mL

Intra-Assay: ≤4.1%

Inter-Assay: ≤7.5%

Recovery: 0, 99

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SEMG2 in samples. An antibody specific for SEMG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: SEMG2 is involved in the formation of a gel matrix that encases ejaculated spermatozoa. Proteolysis by the prostate-specific antigen (PSA) breaks down the gel matrix and allows the spermatozoa to move more freely. The encoded protein is found in lesser abundance than a similar semenogelin protein. The genes encoding these two semenogelin proteins are found in a cluster on chromosome 20. The transcription units are composed of 3 exons, of which the first encodes the signal peptide, the second encodes secreted protein, and the third contains solely 3-prime, noncoding nucleotides. The nucleotide sequences exhibited a similarity of close to 90% in the exons and greater than 80% in the introns and flanking nucleotides.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .