Human Sepiapterin reductase (SPR) ELISA Kit | KTE60344

Human Sepiapterin reductase (SPR) ELISA Kit | KTE60344 | Gentaur UK, US & Europe Distribution

Application: This Human Sepiapterin reductase (SPR) ELISA Kit employs a two-site sandwich ELISA to quantitate SPR in samples. An antibody specific for SPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Sepiapterin reductase microplate
• Human Sepiapterin reductase standard
• Human Sepiapterin reductase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Sepiapterin reductase (SPR) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPR. No significant cross-reactivity or interference between Human SPR and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Sepiapterin reductase (7, 8-dihydrobiopterin:NADP+ oxidoreductase; EC 1.1.1.153) catalyzes the NADPH-dependent reduction of various carbonyl substances, including derivatives of pteridines, and belongs to a group of enzymes called aldo-keto reductases. SPR plays an important role in the biosynthesis of tetrahydrobiopterin (BH4) . The clone encoded a protein of 261 amino acids with a calculated molecular mass of 28, 047 Da. The predicted amino acid sequence of human sepiapterin reductase shows 74% identity with the rat enzyme and a striking homology with human carbonyl reductase, estradiol 17-beta-dehydrogenase, and 3-beta-hydroxy-5-ene steroid dehydrogenase, especially in the N-terminal region.

Alternative Names: SPR; SDR38C1; sepiapterin reductase; short chain dehydrogenase/reductase family 38C; member 1

Search name: SPR; SDR38C1; sepiapterin reductase; short chain dehydrogenase/reductase family 38C; member 1

Tag: SPR