Human Serpin B6 (SERPINB6) ELISA Kit | KTE60697

Human Serpin B6 (SERPINB6) ELISA Kit | KTE60697 | Gentaur UK, US & Europe Distribution

Application: This Human Serpin B6 (SERPINB6) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB6 in samples. An antibody specific for SERPINB6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Serpin B6 microplate
• Human Serpin B6 standard
• Human Serpin B6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Serpin B6 (SERPINB6) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINB6. No significant cross-reactivity or interference between Human SERPINB6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PTI is a member of an emerging class of intracellular serpins.On the basis of its interaction with thrombin, through which it was discovered, the inhibitor was operationally named the placental thrombin inhibitor (PTI) . Amino acid sequence comparisons suggested that its reactive center is located at arg341 and cys342, that it lacks a classic N-terminal signal sequence, and that it has the highest degree of similarity to intracellular serine proteinase inhibitors (serpins), such as the human monocyte/neutrophil elastase inhibitor (ELANH2) . Analysis of human tissue mRNA indicated that PTI is expressed widely, with the highest levels in cardiac and skeletal muscle and placenta.Spi3, is developmentally regulated, and that, unlike the situation in the human, Spi3 is absent from the mouse placenta.

Alternative Names: SERPINB6; RP1-90J20.6; CAP; DKFZp686I04222; MGC111370; MSTP057; PI6; PTI; SPI3; OTTHUMP00000017778; OTTHUMP00000017779; OTTHUMP00000017781; OTTHUMP00000017782; protease inhibitor 6 (placental thrombin inhibitor) ;

Search name: SERPINB6; RP1-90J20.6; CAP; DKFZp686I04222; MGC111370; MSTP057; PI6; PTI; SPI3; OTTHUMP00000017778; OTTHUMP00000017779; OTTHUMP00000017781; OTTHUMP00000017782; protease inhibitor 6 (placental thrombin inhibitor) ;

Tag: SERPINB6