Human SETD7 (SET Domain Containing Protein 7) ELISA Kit | ELK6889

Human SETD7 (SET Domain Containing Protein 7) ELISA Kit | ELK6889 | Gentaur UK, US & Europe Distribution

Alternative Names: SET7; SET7/9; Set9; KMT7; Lysine N-methyltransferase 7; Histone H3-K4 methyltransferase SETD7; Histone-lysine N-methyltransferase SETD7

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SET Domain Containing Protein 7 (SETD7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SET Domain Containing Protein 7 (SETD7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SET Domain Containing Protein 7 (SETD7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SET Domain Containing Protein 7 (SETD7) in the samples is then determined by comparing the OD of the samples to the standard curve.