Human SFRP4 (Secreted Frizzled Related Protein 4) ELISA Kit | ELK3266

Human SFRP4 (Secreted Frizzled Related Protein 4) ELISA Kit | ELK3266 | Gentaur UK, US & Europe Distribution

Alternative Names: FRP-4; FRPHE; Frizzled protein, human endometrium

Species: Human

Assay Type: Sandwich

Sensitivity: 25.8 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 4 (SFRP4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 4 (SFRP4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 4 (SFRP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 4 (SFRP4) in the samples is then determined by comparing the OD of the samples to the standard curve.