Human SGCd (Sarcoglycan Delta) ELISA Kit | ELK4751

Human SGCd (Sarcoglycan Delta) ELISA Kit | ELK4751 | Gentaur UK, US & Europe Distribution

Alternative Names: 35DAG; CMD1L; DAGD; SG-delta; SGCDP; SGD; 35kDa Dystrophin-Associated Glycoprotein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.28 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sarcoglycan Delta (SGCd) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sarcoglycan Delta (SGCd) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sarcoglycan Delta (SGCd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sarcoglycan Delta (SGCd) in the samples is then determined by comparing the OD of the samples to the standard curve.