Human SHP (Small Heterodimer Partner) ELISA Kit | ELK4427

Human SHP (Small Heterodimer Partner) ELISA Kit | ELK4427 | Gentaur UK, US & Europe Distribution

Alternative Names: NR0B2; SHP1; Nuclear Receptor Subfamily 0, Group B, Member 2; Orphan nuclear receptor SHP

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Endocrinology;Developmental science;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Heterodimer Partner (SHP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Small Heterodimer Partner (SHP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Heterodimer Partner (SHP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Heterodimer Partner (SHP) in the samples is then determined by comparing the OD of the samples to the standard curve.