Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit | AE19534HU

Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit | AE19534HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SIGLEC10

Alternative Name: MGC126774; PRO940; SIGLEC-10; SLG2; sialic acid binding Ig-like lectin 10 Ig-like lectin 7|siglec-like gene 2

Application: ELISA

Range: 78.12-5000 pg/mL

Sensitivity: 33 pg/mL

Intra-Assay: ≤7.1%

Inter-Assay: ≤10.9%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SIGLEC10 in samples. An antibody specific for SIGLEC10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIGLEC10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: By searching an EST database for sequences resembling CD33 (SIGLEC3) and screening a spleen cDNA library, Munday et al. (2001) isolated a cDNA encoding SIGLEC10. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and 4 C2-set Ig-like domains, 5 potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .