Human Sialic Acid (SA) ELISA Kit | ELK8419

Human Sialic Acid (SA) ELISA Kit | ELK8419 | Gentaur UK, US & Europe Distribution

Alternative Names: SA

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 3.95 μg/mL

Standard: 500 μg/mL

Detection Range: 7.82-500 μg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 2h

Research Area: Signal transduction;Tumor immunity;Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA (Sialic Acid) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA (Sialic Acid) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA (Sialic Acid) in the samples is then determined by comparing the OD of the samples to the standard curve.