Human SIGLEC2 (Sialic Acid Binding Ig Like Lectin 2) ELISA Kit | ELK3458

Human SIGLEC2 (Sialic Acid Binding Ig Like Lectin 2) ELISA Kit | ELK3458 | Gentaur UK, US & Europe Distribution

Alternative Names: CD22; BL-CAM; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; T-cell surface antigen Leu-14

Species: Human

Assay Type: Sandwich

Sensitivity: 1.14 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 2 (SIGLEC2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sialic Acid Binding Ig Like Lectin 2 (SIGLEC2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Binding Ig Like Lectin 2 (SIGLEC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sialic Acid Binding Ig Like Lectin 2 (SIGLEC2) in the samples is then determined by comparing the OD of the samples to the standard curve.