Human SLC39A6 (Solute Carrier Family 39, Member 6) ELISA Kit | ELK4249

Human SLC39A6 (Solute Carrier Family 39, Member 6) ELISA Kit | ELK4249 | Gentaur UK, US & Europe Distribution

Alternative Names: LIV-1; Zinc Transporter Protein 6; Estrogen-regulated protein LIV-1; Zrt- and Irt-like protein 6; Zinc transporter ZIP6

Species: Human

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 39, Member 6 (SLC39A6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Solute Carrier Family 39, Member 6 (SLC39A6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 39, Member 6 (SLC39A6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 39, Member 6 (SLC39A6) in the samples is then determined by comparing the OD of the samples to the standard curve.