Human SLC7A9 (Solute Carrier Family 7, Member 9) ELISA Kit | ELK5106

Human SLC7A9 (Solute Carrier Family 7, Member 9) ELISA Kit | ELK5106 | Gentaur UK, US & Europe Distribution

Alternative Names: CSNU3; Cationic Amino Acid Transporter, y+ System; b (0, +) -type amino acid transporter 1; Glycoprotein-associated amino acid transporter b0, +AT1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 7, Member 9 (SLC7A9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Solute Carrier Family 7, Member 9 (SLC7A9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 7, Member 9 (SLC7A9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 7, Member 9 (SLC7A9) in the samples is then determined by comparing the OD of the samples to the standard curve.