Human Small ubiquitin-related modifier 1 (SUMO1) ELISA Kit | KTE60404

Human Small ubiquitin-related modifier 1 (SUMO1) ELISA Kit | KTE60404 | Gentaur UK, US & Europe Distribution

Application: This Human Small ubiquitin-related modifier 1 (SUMO1) ELISA Kit employs a two-site sandwich ELISA to quantitate SUMO1 in samples. An antibody specific for SUMO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUMO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SUMO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SUMO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Small ubiquitin-related modifier 1 microplate
• Human Small ubiquitin-related modifier 1 standard
• Human Small ubiquitin-related modifier 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Small ubiquitin-related modifier 1 (SUMO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SUMO1. No significant cross-reactivity or interference between Human SUMO1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Small ubiquitin-related modifier 1 is a member of the SUMO protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.Sequence analysis of the corresponding gene, termed UBL1, revealed that the gene encodes a 101-amino acid polypeptide with homology to ubiquitin and other ubiquitin-like proteins.

Alternative Names: SUMO1; DAP-1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; SUMO-1; UBL1; GAP modifying protein 1; SMT3 suppressor of mif two 3 homolog 1; ubiquitin-like 1 (sentrin)

Search name: SUMO1; DAP-1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; SUMO-1; UBL1; GAP modifying protein 1; SMT3 suppressor of mif two 3 homolog 1; ubiquitin-like 1 (sentrin)

Tag: SUMO1