Human SOCS3 (Suppressors Of Cytokine Signaling 3) ELISA Kit | ELK2144

Human SOCS3 (Suppressors Of Cytokine Signaling 3) ELISA Kit | ELK2144 | Gentaur UK, US & Europe Distribution

Alternative Names: ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3

Species: Human

Assay Type: Sandwich

Sensitivity: 6.1 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Immune molecule;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressors Of Cytokine Signaling 3 (SOCS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppressors Of Cytokine Signaling 3 (SOCS3) in the samples is then determined by comparing the OD of the samples to the standard curve.