Human SOD (Superoxide Dismutases) ELISA Kit | ELK5615

Human SOD (Superoxide Dismutases) ELISA Kit | ELK5615 | Gentaur UK, US & Europe Distribution

Alternative Names: Superoxide Dismutases

Species: Human

Assay Type: Sandwich

Sensitivity: 0.066 U/mL

Standard: 10 U/mL

Detection Range: 0.16-10 U/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Hepatology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutases (SOD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Superoxide Dismutases (SOD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutases (SOD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutases (SOD) in the samples is then determined by comparing the OD of the samples to the standard curve.