Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit | KTE60590

Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit | KTE60590 | Gentaur UK, US & Europe Distribution

Application: This Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A9 in samples. An antibody specific for SLC6A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Sodium- and chloride-dependent glycine transporter 1 microplate
• Human Sodium- and chloride-dependent glycine transporter 1 standard
• Human Sodium- and chloride-dependent glycine transporter 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A9. No significant cross-reactivity or interference between Human SLC6A9 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Glycine transport is mediated by 2 sodium-dependent carriers, GLYT1 and GLYT2, that have distinct tissue distributions. While the 3-prime sequences of these 2 cDNAs were identical, the 5-prime noncoding regions and the N termini were completely different. GLYT1b was found only in the white matter of the CNS, while GLYT1a was found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Tissue-specific alternative splicing or alternative promoter usage from a single gene resulted in 2 mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT1a mRNA supported the emerging status of glycine as a supraspinal neurotransmitter and suggested that glycine may function as a chemical messenger outside the CNS

Alternative Names: SLC6A9; DKFZp547A1118; GLYT1; sodium- and chloride-dependent glycine transporter 1; solute carrier family 6 member 9

Search name: SLC6A9; DKFZp547A1118; GLYT1; sodium- and chloride-dependent glycine transporter 1; solute carrier family 6 member 9

Tag: SLC6A9