Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) ELISA Kit | KTE60225

Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) ELISA Kit | KTE60225 | Gentaur UK, US & Europe Distribution

Application: This Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF11A in samples. An antibody specific for TNFRSF11A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF11A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF11A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF11A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Soluble Receptor Activator of Nuclear Factor-kB microplate
• Human Soluble Receptor Activator of Nuclear Factor-kB standard
• Human Soluble Receptor Activator of Nuclear Factor-kB detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFRSF11A. No significant cross-reactivity or interference between Human TNFRSF11A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Soluble Receptor Activator of Nuclear Factor-kB (sRANK) is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which sRANK induces the activation of NF-kappa B and MAPK8/JNK. sRANKr and its ligand are important regulators of the interaction between T cells and dendritic cells. sRANK is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.

Alternative Names: TNFRSF11A; CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER; loss of heterozygosity; 18; chromosomal region 1; osteoclast differentiation factor receptor; receptor activator of nuclear factor-ka

Search name: TNFRSF11A; CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER; loss of heterozygosity; 18; chromosomal region 1; osteoclast differentiation factor receptor; receptor activator of nuclear factor-ka

Tag: TNFRSF11A