Human Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) ELISA Kit | KTE60209

Human Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) ELISA Kit | KTE60209 | Gentaur UK, US & Europe Distribution

Application: This Human Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF10 in samples. An antibody specific for TNFSF10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Soluble tumor necrosis factor-related apoptosis inducing ligand microplate
• Human Soluble tumor necrosis factor-related apoptosis inducing ligand standard
• Human Soluble tumor necrosis factor-related apoptosis inducing ligand detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFSF10. No significant cross-reactivity or interference between Human TNFSF10 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: This protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

Alternative Names: TNFSF10; APO2L; Apo-2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family; member 10; tumor necrosis factor apoptosis-inducing ligand splice var

Search name: TNFSF10; APO2L; Apo-2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family; member 10; tumor necrosis factor apoptosis-inducing ligand splice var

Tag: TNFSF10