Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit | AE19179HU

Human Solute carrier family 15 member 1 (SLC15A1) ELISA Kit | AE19179HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SLC15A1

Alternative Name: RP11-130L10.1; HPECT1; HPEPT1; PEPT1; Caco-2 oligopeptide transporter|bA551M18.1.1 (solute carrier family 15 (oligopeptide transporter) member 1) |intestinal H+/peptide cotransporter|macrophage oligo

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.126 ng/mL

Intra-Assay: ≤7.2%

Inter-Assay: ≤10.7%

Recovery: 1, 05

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SLC15A1 in samples. An antibody specific for SLC15A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC15A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: SLC15A1 encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs.Studies indicated that it transports dipeptides and tripeptides but not free amino acids or peptides with more than 3 amino acid residues and that its driving force for uphill transport requires proton binding and presence of an inside-negative membrane potential. A membrane protein, HTP1, which appeared to be associated with the oligopeptide transporter, had also been cloned

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .