Human SPR (Sepiapterin Reductase) ELISA Kit | ELK3702

Human SPR (Sepiapterin Reductase) ELISA Kit | ELK3702 | Gentaur UK, US & Europe Distribution

Alternative Names: SDR38C1; 7, 8-Dihydrobiopterin:NADP+ Oxidoreductase; Short Chain Dehydrogenase/Reductase Family 38C, Member 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.108 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sepiapterin Reductase (SPR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sepiapterin Reductase (SPR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sepiapterin Reductase (SPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sepiapterin Reductase (SPR) in the samples is then determined by comparing the OD of the samples to the standard curve.