Human SRSF2 (Serine/Arginine Rich Splicing Factor 2) ELISA Kit | ELK3310

Human SRSF2 (Serine/Arginine Rich Splicing Factor 2) ELISA Kit | ELK3310 | Gentaur UK, US & Europe Distribution

Alternative Names: SFRS2; PR264; SC-35; SC35; SFRS2A; SRp30b; Splicing component, 35 kDa; Splicing factor, arginine/serine-rich 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Arginine Rich Splicing Factor 2 (SRSF2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serine/Arginine Rich Splicing Factor 2 (SRSF2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Arginine Rich Splicing Factor 2 (SRSF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Arginine Rich Splicing Factor 2 (SRSF2) in the samples is then determined by comparing the OD of the samples to the standard curve.