855

Human SUMO2 (Small Ubiquitin Related Modifier Protein 2) ELISA Kit | ELK3744

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SKU:
855-ELK3744-GEN
NULL588.00 - NULL704.00

Description

Human SUMO2 (Small Ubiquitin Related Modifier Protein 2) ELISA Kit | ELK3744 | Gentaur UK, US & Europe Distribution

Alternative Names: HSMT3; SMT3B; SMT3H2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3B

Species: Human

Assay Type: Sandwich

Sensitivity: 0.126 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 2 (SUMO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Ubiquitin Related Modifier Protein 2 (SUMO2) in the samples is then determined by comparing the OD of the samples to the standard curve.

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