855
Human TACTILE (T-Cell Activation Antigen, Increased Late Expression) ELISA Kit | ELK5595
- SKU:
- 855-ELK5595-GEN
Description
Human TACTILE (T-Cell Activation Antigen, Increased Late Expression) ELISA Kit | ELK5595 | Gentaur UK, US & Europe Distribution
Alternative Names: CD96; T-cell surface protein tactile
Species: Human
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Activation Antigen, Increased Late Expression (TACTILE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T-Cell Activation Antigen, Increased Late Expression (TACTILE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Activation Antigen, Increased Late Expression (TACTILE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell Activation Antigen, Increased Late Expression (TACTILE) in the samples is then determined by comparing the OD of the samples to the standard curve.