Human TAF13 (TATA Box Binding Protein Associated Factor 13) ELISA Kit | ELK3962

Human TAF13 (TATA Box Binding Protein Associated Factor 13) ELISA Kit | ELK3962 | Gentaur UK, US & Europe Distribution

Alternative Names: TAF2K; TAFII18; Transcription Initiation Factor TFIID Subunit 13; Transcription initiation factor TFIID 18 kDa subunit

Species: Human

Assay Type: Sandwich

Sensitivity: 0.128 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TATA Box Binding Protein Associated Factor 13 (TAF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TATA Box Binding Protein Associated Factor 13 (TAF13) in the samples is then determined by comparing the OD of the samples to the standard curve.