Human TAP1 (Antigen Peptide Transporter 1) ELISA Kit | ELK4394

Human TAP1 (Antigen Peptide Transporter 1) ELISA Kit | ELK4394 | Gentaur UK, US & Europe Distribution

Alternative Names: APT1; ABC17; ABCB2; PSF1; RING4; Y3; TAP1N; Peptide Supply Factor 1; Atp-Binding Cassette, Subfamily B, Member 2; Peptide transporter involved in antigen processing 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.134 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Antigen Peptide Transporter 1 (TAP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Antigen Peptide Transporter 1 (TAP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Antigen Peptide Transporter 1 (TAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Antigen Peptide Transporter 1 (TAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.