Human TAT (Thrombin/Antithrombin Complex) ELISA Kit | ELK1049

Human TAT (Thrombin/Antithrombin Complex) ELISA Kit | ELK1049 | Gentaur UK, US & Europe Distribution

Alternative Names: Thrombin/Antithrombin Complex

Species: Human

Assay Type: Sandwich

Sensitivity: 2.87 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: Plasma

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombin/Antithrombin Complex (TAT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thrombin/Antithrombin Complex (TAT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombin/Antithrombin Complex (TAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombin/Antithrombin Complex (TAT) in the samples is then determined by comparing the OD of the samples to the standard curve.