Human Tenascin-X (TNXB) ELISA Kit | KTE62316

Human Tenascin-X (TNXB) ELISA Kit | KTE62316 | Gentaur UK, US & Europe Distribution

Application: This Human Tenascin-X (TNXB) ELISA Kit employs a two-site sandwich ELISA to quantitate TNXB in samples. An antibody specific for TNXB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNXB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNXB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNXB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Tenascin-X microplate
• Human Tenascin-X standard
• Human Tenascin-X detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Tenascin-X (TNXB) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNXB. No significant cross-reactivity or interference between Human TNXB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Tenascin X is a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternative Names: TNXB; DAAP-193O6.1; HXBL; TENX; TNX; TNXB1; TNXB2; TNXBS; XB; XBS; growth-inhibiting protein 45; hexabrachion-like; tenascin XB1; tenascin XB2; tenascin-X

Search name: TNXB; DAAP-193O6.1; HXBL; TENX; TNX; TNXB1; TNXB2; TNXBS; XB; XBS; growth-inhibiting protein 45; hexabrachion-like; tenascin XB1; tenascin XB2; tenascin-X

Tag: TNXB