Human TERF2IP (Telomeric Repeat Binding Factor 2 Interacting Protein) ELISA Kit | ELK4298

Human TERF2IP (Telomeric Repeat Binding Factor 2 Interacting Protein) ELISA Kit | ELK4298 | Gentaur UK, US & Europe Distribution

Alternative Names: RAP1; DRIP5; Dopamine receptor-interacting protein 5; Repressor/activator protein 1 homolog

Species: Human

Assay Type: Sandwich

Sensitivity: 0.66 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) in the samples is then determined by comparing the OD of the samples to the standard curve.