Human Thrombin activatable fibrinolysis inhibitor (TAFI) ELISA Kit | KTE62275

Human Thrombin activatable fibrinolysis inhibitor (TAFI) ELISA Kit | KTE62275 | Gentaur UK, US & Europe Distribution

Application: This Human Thrombin activatable fibrinolysis inhibitor (TAFI) ELISA Kit employs a two-site sandwich ELISA to quantitate CPB2 in samples. An antibody specific for CPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Thrombin activatable fibrinolysis inhibitor microplate
• Human Thrombin activatable fibrinolysis inhibitor standard
• Human Thrombin activatable fibrinolysis inhibitor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Thrombin activatable fibrinolysis inhibitor (TAFI) ELISA Kit has high sensitivity and excellent specificity for detection of Human CPB2. No significant cross-reactivity or interference between Human CPB2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TAFI, Thrombin Activatable Fibrinolytic Inhibitor, (also known as carboxypeptidase U and plasma carboxypeptidase B) is a 60, 000 D molecular ratio glycoprotein (proenzyme form) present in rabbit plasma1 that modulates fibrinolysis in vivo. This proenzyme is converted to a 35, 000 D molecular ratio active form, TAFIa, following proteolytic cleavage by the thrombin/ thrombomodulin complex. TAFIa possesses carboxypeptidase activity with a preference for cleaving lysine and arginine residues from the c-terminus of proteins. Modulation of fibrinolysis occurs when TAFIa cleaves C-terminal arginine and lysine residues of partially degraded fibrin.2, 4, 5 The removal of the c-terminus arginine and lysine residues from fibrin inhibits the continued degradation of fibrin by tPA activated plasmin.3

Alternative Names: CPB2; CPU; PCPB; TAFI; carboxypeptidase B-like protein; carboxypeptidase B2 (plasma; carboxypeptidase U) ; carboxypeptidase U; plasma carboxypeptidase B2; thrombin-activable fibrinolysis inhibitor; thrombin-act

Search name: CPB2; CPU; PCPB; TAFI; carboxypeptidase B-like protein; carboxypeptidase B2 (plasma; carboxypeptidase U) ; carboxypeptidase U; plasma carboxypeptidase B2; thrombin-activable fibrinolysis inhibitor; thrombin-act

Tag: CPB2