Human Tissue-type plasminogen activator (PLAT) ELISA Kit | KTE62730

Human Tissue-type plasminogen activator (PLAT) ELISA Kit | KTE62730 | Gentaur UK, US & Europe Distribution

Application: This Human Tissue-type plasminogen activator (PLAT) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAT in samples. An antibody specific for PLAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Tissue-type plasminogen activator microplate
• Human Tissue-type plasminogen activator standard
• Human Tissue-type plasminogen activator detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Tissue-type plasminogen activator (PLAT) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLAT. No significant cross-reactivity or interference between Human PLAT and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Tissue plasminogen activator is a protein involved in the breakdown of blood clots. Specifically, it is a serine protease (EC 3.4.21.68) found on endothelial cells, the cells that line the blood vessels. As an enzyme, it catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown. Because it works on the clotting system, tPA is used in clinical medicine to treat only embolic or thrombolytic stroke. Use is contraindicated in hemorrhagic stroke and head trauma.The classic role of tPA is in the clotting system. Specifically, tPA catalyzes the conversion of plasminogen into plasmin. It does so by cleaving the single-chained plasminogen into two chains. These two chains are linked by a disulfide bond and the resulting molecule is called plasmin.

Alternative Names: PLAT; DKFZp686I03148; T-PA; TPA; alteplase; plasminogen activator; tissue type; reteplase; t-plasminogen activator; tissue plasminogen activator (t-PA)

Search name: PLAT; DKFZp686I03148; T-PA; TPA; alteplase; plasminogen activator; tissue type; reteplase; t-plasminogen activator; tissue plasminogen activator (t-PA)

Tag: PLAT