Human TNF-α protein | PRP1013

Human TNF-α protein | PRP1013 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.

Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/ml.

protein Lenght: The recombinant human TNF-α consists of 161 amino acids and has a predicted molecular mass of 17.7 kDa which is also estimated by SDS-PAGE.

Preparation: E. coli

Purity: > 95 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile 150mM NaCl 20mM Tris, pH 8.0.

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 17.7 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human TNF-α protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human TNF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.2% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Tumor necrosis factor α (TNF-α), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-α. TNF-α protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-α is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Alternative Names: DIF; TNF-α; TNFA; TNFSF2; Tumor necrosis factor; TNF

Search Names: DIF; TNF-α; TNFA; TNFSF2; Tumor necrosis factor; TNF

Tag: TNF-α