Human TPM3 (Tropomyosin 3) ELISA Kit | ELK4088

Human TPM3 (Tropomyosin 3) ELISA Kit | ELK4088 | Gentaur UK, US & Europe Distribution

Alternative Names: NEM1; TRK; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TPMsk3; Hscp30; Gamma-tropomyosin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tropomyosin 3 (TPM3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tropomyosin 3 (TPM3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tropomyosin 3 (TPM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tropomyosin 3 (TPM3) in the samples is then determined by comparing the OD of the samples to the standard curve.