Human TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit | ELK9058

Human TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit | ELK9058 | Gentaur UK, US & Europe Distribution

Alternative Names: TPO Ab; TPO-Ab

Species: Human

Assay Type: Sandwich

Sensitivity: 1.19 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to anti-Thyroid-Peroxidase antibody (TPO-Ab) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-Thyroid-Peroxidase antibody (TPO-Ab) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain anti-Thyroid-Peroxidase antibody (TPO-Ab), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of anti-Thyroid-Peroxidase antibody (TPO-Ab) in the samples is then determined by comparing the OD of the samples to the standard curve.