Human TRA2b (Transformer 2 Beta) ELISA Kit | ELK7602

Human TRA2b (Transformer 2 Beta) ELISA Kit | ELK7602 | Gentaur UK, US & Europe Distribution

Alternative Names: SRFS10; Htra2-beta; TRA2-BETA; TRA2B; Splicing Factor, Arginine/Serine-Rich 10; Transformer-2 protein homolog B

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transformer 2 Beta (TRA2b) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transformer 2 Beta (TRA2b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transformer 2 Beta (TRA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transformer 2 Beta (TRA2b) in the samples is then determined by comparing the OD of the samples to the standard curve.