Human TRAF2 (TNF Receptor-Associated Factor 2) ELISA Kit | ELK8544

Human TRAF2 (TNF Receptor-Associated Factor 2) ELISA Kit | ELK8544 | Gentaur UK, US & Europe Distribution

Alternative Names: TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3

Species: Human

Assay Type: Sandwich

Sensitivity: 25.1 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor-Associated Factor 2 (TRAF2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNF Receptor-Associated Factor 2 (TRAF2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor-Associated Factor 2 (TRAF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF Receptor-Associated Factor 2 (TRAF2) in the samples is then determined by comparing the OD of the samples to the standard curve.