Human Transcriptional repressor protein YY1 (YY1) ELISA Kit | KTE60006

Human Transcriptional repressor protein YY1 (YY1) ELISA Kit | KTE60006 | Gentaur UK, US & Europe Distribution

Application: This Human Transcriptional repressor protein YY1 (YY1) ELISA Kit employs a two-site sandwich ELISA to quantitate YY1 in samples. An antibody specific for YY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Transcriptional repressor protein YY1 microplate
• Human Transcriptional repressor protein YY1 standard
• Human Transcriptional repressor protein YY1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Transcriptional repressor protein YY1 (YY1) ELISA Kit has high sensitivity and excellent specificity for detection of Human YY1. No significant cross-reactivity or interference between Human YY1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins.

Alternative Names: YY1; DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1; INO80 complex subunit S; Yin and Yang 1 protein

Search name: YY1; DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1; INO80 complex subunit S; Yin and Yang 1 protein

Tag: YY1