Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit | KTE60149

Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit | KTE60149 | Gentaur UK, US & Europe Distribution

Application: This Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit employs a two-site sandwich ELISA to quantitate TPM3 in samples. An antibody specific for TPM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Tropomyosin alpha-3 chain microplate
• Human Tropomyosin alpha-3 chain standard
• Human Tropomyosin alpha-3 chain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TPM3. No significant cross-reactivity or interference between Human TPM3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Tropomyosin alpha-3 chain is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternative Names: TPM3; RP11-205M9.1; FLJ41118; MGC14582; MGC3261; MGC72094; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TPMsk3; TRK; hscp30; cytoskeletal tropomyosin TM30; heat-stable cytoskeletal protein 30 kDa; tropomyosin

Search name: TPM3; RP11-205M9.1; FLJ41118; MGC14582; MGC3261; MGC72094; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TPMsk3; TRK; hscp30; cytoskeletal tropomyosin TM30; heat-stable cytoskeletal protein 30 kDa; tropomyosin

Tag: TPM3