Human TSPO (Translocator Protein) ELISA Kit | ELK3592

Human TSPO (Translocator Protein) ELISA Kit | ELK3592 | Gentaur UK, US & Europe Distribution

Alternative Names: IBP; PKBS; PBR; pk18; mDRC; MBR; BZRP; DBI; PTBR; Mitochondrial benzodiazepine receptor; Peripheral-Type Benzodiazepine Receptor/Recognition Site

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocator Protein (TSPO) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Translocator Protein (TSPO) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocator Protein (TSPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocator Protein (TSPO) in the samples is then determined by comparing the OD of the samples to the standard curve.