Human Tubulin Beta-3 Chain (TUBB3) ELISA Kit | AE59490HU

Human Tubulin Beta-3 Chain (TUBB3) ELISA Kit | AE59490HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: TUBB3

Alternative Name: MC1R; TUBB4; beta-4; tubulin; beta; 4

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.122 ng/mL

Intra-Assay: ≤5.8%

Inter-Assay: ≤10.8%

Recovery: 0, 95

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TUBB3 in samples. An antibody specific for TUBB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUBB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUBB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUBB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: TUBB3 expression in several human cell lines, but not in a paclitaxel-resistant cell line. RNA interference revealed that HIF1A mediated hypoxia-induced TUBB3 expression. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression. Much lower Tubb3 levels were detected in all other adult mouse tissues examined except brain, where expression was even lower. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .