Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit | AE13642HU

Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit | AE13642HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: TPPP

Alternative Name: TPPP/p25; TPPP1; p24; p25; p25alpha; brain specific protein p25 alpha|glycogen synthase kinase 3 (GSK3) inhibitor p24|tubulin polymerization-promoting protein

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.128 ng/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤7.3%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TPPP in samples. An antibody specific for TPPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPPP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Seki et al. (1999) cloned p25 from a neuroblastoma cDNA library. The deduced 219-amino acid protein has a calculated molecular mass of about 24 kD. Human and bovine p25 share 90% amino acid identity. In contrast to the brain-specific expression of bovine p25, RT-PCR detected human p25 in all tissues examined.Martin et al. (2002) found that p24 associated with Gsk3 in microtubules from adult rat brain. p24 bound Gsk3 and inhibited its kinase activity at low magnesium concentrations. p24 was a poor substrate for Gsk3, but it could be phosphorylated by other protein kinases. Bovine Tppp inhibited mitotic spindle assembly and nuclear envelope breakdown following injection into Drosophila embryos. It did not affect other cellular events.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .