Human UBD (Ubiquitin D) ELISA Kit | ELK2165

Human UBD (Ubiquitin D) ELISA Kit | ELK2165 | Gentaur UK, US & Europe Distribution

Alternative Names: UB-D; FAT10; UBD-3; Diubiquitin; Ubiquitin-like protein FAT10

Species: Human

Assay Type: Sandwich

Sensitivity: 1.34 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids. 

Assay Lenght: 3.5h

Research Area: Infection immunity;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin D (UBD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ubiquitin D (UBD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin D (UBD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin D (UBD) in the samples is then determined by comparing the OD of the samples to the standard curve.