Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit | AE12449HU

Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit | AE12449HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: UBE3A

Alternative Name: ANCR; AS; E6-AP; EPVE6AP; FLJ26981; HPVE6A; CTCL tumor antigen se37-2|human papilloma virus E6-associated protein|oncogenic protein-associated protein E6-AP

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.114 ng/mL

Intra-Assay: ≤4.8%

Inter-Assay: ≤7.6%

Recovery: 0, 96

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate UBE3A in samples. An antibody specific for UBE3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Ubiquitin-protein ligase E3A (UBE3A) is an enzyme that in humans is encoded by the UBE3A gene. This enzyme is involved in targeting proteins for degradation within cells. Protein degradation is a normal process that removes damaged or unnecessary proteins and helps maintain the normal functions of cells. Ubiquitin protein ligase 3A attaches a small protein called ubiquitin to proteins that should be degraded. Cellular structures called proteasomes recognize and digest proteins tagged with ubiquitin. Both copies of the UBE3A gene are active in most of the body's tissues. In the brain, however, only the copy inherited from a person's mother (the maternal copy) is normally active. The UBE3A gene is located on the long (q) arm of chromosome 15 between positions 11 and 13, from base pair 23, 133, 488 to base pair 23, 235, 220.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .