Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit | AE11884HU

Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit | AE11884HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: UXS1

Alternative Name: FLJ23591; SDR6E1; UGD; short chain dehydrogenase/reductase family 6E; member 12

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.116 ng/mL

Intra-Assay: ≤4.6%

Inter-Assay: ≤8.3%

Recovery: 0, 83

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate UXS1 in samples. An antibody specific for UXS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUXS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UXS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UXS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: UDP-glucuronate decarboxylase (UGD; EC 4.1.1.35) catalyzes the formation of UDP-xylose from UDP-glucuronate. UDP-xylose is then used to initiate glycosaminoglycan biosynthesis on the core protein of proteoglycans. The topology of the deduced 420-amino acid protein was consistent with a type II transmembrane protein. Northern blot analysis of rat tissues detected a single UGD transcript with highest levels in heart, brain, and testes. Substantial levels were also detected in kidney, liver and lung, and lower levels in spleen and skeletal muscle. Western blot analysis demonstrated highest levels of the protein in kidney, liver, and brain, with negligible staining in heart, spleen, skeletal muscle, lung, and testes. Subcellular studies and histochemistry localized the protein to the perinuclear Golgi.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .