Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) ELISA Kit | KTE61988

Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) ELISA Kit | KTE61988 | Gentaur UK, US & Europe Distribution

Application: This Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DPAGT1 in samples. An antibody specific for DPAGT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPAGT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPAGT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPAGT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase microplate
• Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase standard
• Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPAGT1. No significant cross-reactivity or interference between Human DPAGT1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DPAGT1 is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. The cDNA encodes a deduced 400-amino acid protein with a calculated molecular mass of 44.7 kD. DPAGT1 contains an N-terminal signal peptide, 2 potential dolichol-binding sequences, and 4 sites for N-glycosylation. It shares 93% amino acid homology with hamster Dpagt, including 100% identity in the dolichol-binding region, and 42% homology with S. cerevisiae GlcNAc-1-P transferase.

Alternative Names: DPAGT1; ALG7; CDG-Ij; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT; GlcNAc-1-P transferase; N-acetylglucosamine-1-phosphate transferase; OTTHUMP00000204055; UDP-GlcNAc:dolichyl-phosphate N-acetylglucosa

Search name: DPAGT1; ALG7; CDG-Ij; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT; GlcNAc-1-P transferase; N-acetylglucosamine-1-phosphate transferase; OTTHUMP00000204055; UDP-GlcNAc:dolichyl-phosphate N-acetylglucosa

Tag: DPAGT1