Human VEGFB (Vascular Endothelial Growth Factor B) ELISA Kit | ELK2321

Human VEGFB (Vascular Endothelial Growth Factor B) ELISA Kit | ELK2321 | Gentaur UK, US & Europe Distribution

Alternative Names: VEGF-B; VEGFL; VRF; VEGF-Related Factor

Species: Human

Assay Type: Sandwich

Sensitivity: 5.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor B (VEGFB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor B (VEGFB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor B (VEGFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Endothelial Growth Factor B (VEGFB) in the samples is then determined by comparing the OD of the samples to the standard curve.