Human Vitamin K1 (VK1) ELISA Kit | KTE60052

Human Vitamin K1 (VK1) ELISA Kit | KTE60052 | Gentaur UK, US & Europe Distribution

Application: This Human Vitamin K1 (VK1) ELISA Kit employs a two-site sandwich ELISA to quantitate VK1 in samples. An antibody specific for VK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Vitamin K1 microplate
• Human Vitamin K1 standard
• Human Vitamin K1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Vitamin K1 (VK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VK1. No significant cross-reactivity or interference between Human VK1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Phylloquinone is a polycyclic aromatic ketone, based on 2-methyl-1, 4-naphthoquinone, with a 3-phytyl substituent.It is a fat-soluble vitamin that is stable to air and moisture but decomposes in sunlight. It is found naturally in a wide variety of green plants.It is often called vitamin K1 or phytonadione. Sometimes a distinction is made with phylloquinone considered natural and phytonadione considered synthetic. A stereoisomer of phylloquinone is called vitamin k1 .Its best known mechanism of action is as a cofactor in the formation of coagulation factors II (prothrombin), VII, IX and X by the liver. Vitamin K1 is commonly used to treat warfarin toxicity, and an antidote for coumatetralyl. It is also required for the formation of anticoagulant factors protein C and S. Vitamin K is also required bone protein formation.

Alternative Names: VK1; Phylloquinone; Phytonadione; Phytomenadione

Search name: VK1; Phylloquinone; Phytonadione; Phytomenadione

Tag: VK1