Human VLDL (Very Low Density Lipoprotein) ELISA Kit | ELK2935

Human VLDL (Very Low Density Lipoprotein) ELISA Kit | ELK2935 | Gentaur UK, US & Europe Distribution

Alternative Names: Pre-β-Lipoprotein; Pre-Beta Lipoprotein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.11 ng/mL

Standard: 15 ng/mL

Detection Range: 0.24-15 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Very Low Density Lipoprotein (VLDL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Very Low Density Lipoprotein (VLDL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Very Low Density Lipoprotein (VLDL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Very Low Density Lipoprotein (VLDL) in the samples is then determined by comparing the OD of the samples to the standard curve.