Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit | KTE60043

Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit | KTE60043 | Gentaur UK, US & Europe Distribution

Application: This Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate VWA3A in samples. An antibody specific for VWA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVWA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VWA3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VWA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Von Willebrand factor A domain-containing protein 3A microplate
• Human Von Willebrand factor A domain-containing protein 3A standard
• Human Von Willebrand factor A domain-containing protein 3A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human VWA3A. No significant cross-reactivity or interference between Human VWA3A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: vWA3A, Contains 2 VWFA domains, Human WARP shares 79% amino acid identity with mouse Warp. RT-PCR of mRNA from various mouse tissues and cell lines detected Warp expression only in chondrocytes. Northern blot analysis confirmed expression of Warp in chondrocytes isolated from newborn mouse rib cartilage. Western blot analysis of transfected human embryonic kidney cells detected a 48-kD protein in both cell layer and media fractions. The majority of the protein was detected in the medium, indicating that WARP is efficiently secreted. Under nonreducing conditions, a 102-kD form was detected, suggesting that WARP forms a disulfide-bonded homodimer. N-glycosidase digestion led to a mobility shift from 48 to 45 kD, indicating that WARP has 1 or more N-linked oligosaccharide chains.

Alternative Names: VWA3A; hCG_2039627; FLJ40941; FLJ46765;

Search name: VWA3A; hCG_2039627; FLJ40941; FLJ46765;

Tag: VWA3A