Human vWA3A (Von Willebrand Factor A Domain Containing Protein 3A) ELISA Kit | ELK1842

Human vWA3A (Von Willebrand Factor A Domain Containing Protein 3A) ELISA Kit | ELK1842 | Gentaur UK, US & Europe Distribution

Alternative Names: VWA3A

Species: Human

Assay Type: Sandwich

Sensitivity: 0.15 ng/mL

Standard: 30 ng/mL

Detection Range: 0.47-30 ng/mL

Sample Type: Plasma

Assay Lenght: 3.5h

Research Area: Developmental science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Willebrand Factor A Domain Containing Protein 3A (vWA3A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Von Willebrand Factor A Domain Containing Protein 3A (vWA3A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Willebrand Factor A Domain Containing Protein 3A (vWA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Willebrand Factor A Domain Containing Protein 3A (vWA3A) in the samples is then determined by comparing the OD of the samples to the standard curve.